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Cambrex Corporation
The PKLight™ HTS Protein Kinase Assay Kit is a patented generic, homogeneous assay designed for use in high throughput screening of kinase activity. The kit contains all reagents necessary for the assay and has been designed for use in a cell free system with either 96, 384 or 1536 well microtiter plates. All that is required from the user is a purified form of the kinase of interest, a suitable substrate, buffer and ATP. The PKLight Assay can be easily optimized for each kinase/substrate pair to produce rapid, reproducible data suitable for IC50 determinations of screen compounds. This technology does not require radioactive beads, radio-labeled ATP, specially designed antibodies or specifically modified substrate sequences.
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